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e coli p aeruginosa shuttle vector  (ATCC)


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    Structured Review

    ATCC e coli p aeruginosa shuttle vector
    Schematic depiction of reporter plasmid and fluorescence gene induction by the β-lactam antibiotic. (A) The plasmid map for pCN61_AmpRtdT is shown to the left. Ampicillin (AP, Tem-1 β-lactamase) or streptomycin (STR) is used for selection. The ampRC operon (top right) and the reporter operon where tdTomato is positioned downstream of the ampC promoter (denoted PampC) are depicted. (B) We constructed GFP-labeled P. <t>aeruginosa</t> and transformed this bacterium with pCN61_AmpRtdT. The bacteria were inoculated on an agar plate in proximity to the β-lactam antibiotic CAZ (spotted at the red dot). The antibiotic diffuses outward from the spot. The interface (white arrow) of CAZ and the GFP-labeled P. aeruginosa without the plasmid shows only green fluorescence (top row). In contrast, the GFP-labeled P. aeruginosa transformed by pCN61_AmpRtdT shows green fluorescence (constituitively) and the red fluorescence induced by the cell-wall-acting antibiotic (bottom row). The top row confirms the absence of red backgroud fluorescence from P. aeruginosa. The bottom row demonstrates the functionality of our reporter plasmid. A 10 μM scale bar is given in panel two of the top row.
    E Coli P Aeruginosa Shuttle Vector, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e+coli+p+aeruginosa+shuttle+vector/pmc07980316-259-19-27?v=ATCC
    Average 93 stars, based on 27 article reviews
    e coli p aeruginosa shuttle vector - by Bioz Stars, 2026-07
    93/100 stars

    Images

    1) Product Images from "Fluorescence Assessment of the AmpR-Signaling Network of Pseudomonas aeruginosa to Exposure to β ‑Lactam Antibiotics"

    Article Title: Fluorescence Assessment of the AmpR-Signaling Network of Pseudomonas aeruginosa to Exposure to β ‑Lactam Antibiotics

    Journal: ACS chemical biology

    doi: 10.1021/acschembio.9b00875

    Schematic depiction of reporter plasmid and fluorescence gene induction by the β-lactam antibiotic. (A) The plasmid map for pCN61_AmpRtdT is shown to the left. Ampicillin (AP, Tem-1 β-lactamase) or streptomycin (STR) is used for selection. The ampRC operon (top right) and the reporter operon where tdTomato is positioned downstream of the ampC promoter (denoted PampC) are depicted. (B) We constructed GFP-labeled P. aeruginosa and transformed this bacterium with pCN61_AmpRtdT. The bacteria were inoculated on an agar plate in proximity to the β-lactam antibiotic CAZ (spotted at the red dot). The antibiotic diffuses outward from the spot. The interface (white arrow) of CAZ and the GFP-labeled P. aeruginosa without the plasmid shows only green fluorescence (top row). In contrast, the GFP-labeled P. aeruginosa transformed by pCN61_AmpRtdT shows green fluorescence (constituitively) and the red fluorescence induced by the cell-wall-acting antibiotic (bottom row). The top row confirms the absence of red backgroud fluorescence from P. aeruginosa. The bottom row demonstrates the functionality of our reporter plasmid. A 10 μM scale bar is given in panel two of the top row.
    Figure Legend Snippet: Schematic depiction of reporter plasmid and fluorescence gene induction by the β-lactam antibiotic. (A) The plasmid map for pCN61_AmpRtdT is shown to the left. Ampicillin (AP, Tem-1 β-lactamase) or streptomycin (STR) is used for selection. The ampRC operon (top right) and the reporter operon where tdTomato is positioned downstream of the ampC promoter (denoted PampC) are depicted. (B) We constructed GFP-labeled P. aeruginosa and transformed this bacterium with pCN61_AmpRtdT. The bacteria were inoculated on an agar plate in proximity to the β-lactam antibiotic CAZ (spotted at the red dot). The antibiotic diffuses outward from the spot. The interface (white arrow) of CAZ and the GFP-labeled P. aeruginosa without the plasmid shows only green fluorescence (top row). In contrast, the GFP-labeled P. aeruginosa transformed by pCN61_AmpRtdT shows green fluorescence (constituitively) and the red fluorescence induced by the cell-wall-acting antibiotic (bottom row). The top row confirms the absence of red backgroud fluorescence from P. aeruginosa. The bottom row demonstrates the functionality of our reporter plasmid. A 10 μM scale bar is given in panel two of the top row.

    Techniques Used: Plasmid Preparation, Fluorescence, Selection, Construct, Labeling, Transformation Assay

    Correlation of the β-lactamase and fluorescence assays. (A) Nitrocefin hydrolysis by AmpC β-lactamase shifts λmax from 390 nm (yellow) to 486 nm (red). (B) Nitrocefin assay performed for P. aeruginosa wild-type and dacB::Tn is plotted as the slope of the absorbance at 486 nm over time for two concentrations of FOX (1/8 MIC and 1/4 MIC) and no antibiotic. (C) Fluorescent response expressed as relative fluorescence (A.U.) of P. aeruginosa wild-type and dacB::Tn under the same experimental conditions as used for the nitrocefin assay. The error bars represent the standard deviation of three biological replicates.
    Figure Legend Snippet: Correlation of the β-lactamase and fluorescence assays. (A) Nitrocefin hydrolysis by AmpC β-lactamase shifts λmax from 390 nm (yellow) to 486 nm (red). (B) Nitrocefin assay performed for P. aeruginosa wild-type and dacB::Tn is plotted as the slope of the absorbance at 486 nm over time for two concentrations of FOX (1/8 MIC and 1/4 MIC) and no antibiotic. (C) Fluorescent response expressed as relative fluorescence (A.U.) of P. aeruginosa wild-type and dacB::Tn under the same experimental conditions as used for the nitrocefin assay. The error bars represent the standard deviation of three biological replicates.

    Techniques Used: Fluorescence, Standard Deviation

    GFP-labeled P. aeruginosa harboring pCN61_AmpRtdT was imaged on a swarm plate in proximity of a second bacterium, either (A) P. mesacidophila, (B) B. licheniformis, (C) E. coli, or (D) M. xanthus. Each panel depicts the bacteria after 15 h of growth. P. aeruginosa is to the left of each plate, while the second bacterium is to the right (see plate panels). The white arrow (top plate) points to a representative site where the two bacteria encounter one another. The second column from left shows bright-field images of comingled bacteria, where the strains meet, as uniform continuous lawns. The GFP panel shows the green fluorescence displayed by P. aeruginosa in this lawn. The black voids are the locations of the second bacterium in this same lawn. The RFP panel shows the red-fluorescent signal from P. aeruginosa that results from contact with an antibiotic-producer strain. Red fluorescence is seen for P. mesacidophila and B. lichemiformis as antibiotic producers. No red fluorescence is seen for E. coli and M. xanthus (not antibiotic producers). The far-right column merges the green and red fluorescent images. A 10-μm black scale bar is given in the bright-field image of panel A.
    Figure Legend Snippet: GFP-labeled P. aeruginosa harboring pCN61_AmpRtdT was imaged on a swarm plate in proximity of a second bacterium, either (A) P. mesacidophila, (B) B. licheniformis, (C) E. coli, or (D) M. xanthus. Each panel depicts the bacteria after 15 h of growth. P. aeruginosa is to the left of each plate, while the second bacterium is to the right (see plate panels). The white arrow (top plate) points to a representative site where the two bacteria encounter one another. The second column from left shows bright-field images of comingled bacteria, where the strains meet, as uniform continuous lawns. The GFP panel shows the green fluorescence displayed by P. aeruginosa in this lawn. The black voids are the locations of the second bacterium in this same lawn. The RFP panel shows the red-fluorescent signal from P. aeruginosa that results from contact with an antibiotic-producer strain. Red fluorescence is seen for P. mesacidophila and B. lichemiformis as antibiotic producers. No red fluorescence is seen for E. coli and M. xanthus (not antibiotic producers). The far-right column merges the green and red fluorescent images. A 10-μm black scale bar is given in the bright-field image of panel A.

    Techniques Used: Labeling, Fluorescence



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    e coli p aeruginosa shuttle vector  (ATCC)
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    ATCC e coli p aeruginosa shuttle vector
    Schematic depiction of reporter plasmid and fluorescence gene induction by the β-lactam antibiotic. (A) The plasmid map for pCN61_AmpRtdT is shown to the left. Ampicillin (AP, Tem-1 β-lactamase) or streptomycin (STR) is used for selection. The ampRC operon (top right) and the reporter operon where tdTomato is positioned downstream of the ampC promoter (denoted PampC) are depicted. (B) We constructed GFP-labeled P. <t>aeruginosa</t> and transformed this bacterium with pCN61_AmpRtdT. The bacteria were inoculated on an agar plate in proximity to the β-lactam antibiotic CAZ (spotted at the red dot). The antibiotic diffuses outward from the spot. The interface (white arrow) of CAZ and the GFP-labeled P. aeruginosa without the plasmid shows only green fluorescence (top row). In contrast, the GFP-labeled P. aeruginosa transformed by pCN61_AmpRtdT shows green fluorescence (constituitively) and the red fluorescence induced by the cell-wall-acting antibiotic (bottom row). The top row confirms the absence of red backgroud fluorescence from P. aeruginosa. The bottom row demonstrates the functionality of our reporter plasmid. A 10 μM scale bar is given in panel two of the top row.
    E Coli P Aeruginosa Shuttle Vector, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e+coli+p+aeruginosa+shuttle+vector/pmc07980316-259-19-27?v=ATCC
    Average 93 stars, based on 1 article reviews
    e coli p aeruginosa shuttle vector - by Bioz Stars, 2026-07
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Schematic depiction of reporter plasmid and fluorescence gene induction by the β-lactam antibiotic. (A) The plasmid map for pCN61_AmpRtdT is shown to the left. Ampicillin (AP, Tem-1 β-lactamase) or streptomycin (STR) is used for selection. The ampRC operon (top right) and the reporter operon where tdTomato is positioned downstream of the ampC promoter (denoted PampC) are depicted. (B) We constructed GFP-labeled P. aeruginosa and transformed this bacterium with pCN61_AmpRtdT. The bacteria were inoculated on an agar plate in proximity to the β-lactam antibiotic CAZ (spotted at the red dot). The antibiotic diffuses outward from the spot. The interface (white arrow) of CAZ and the GFP-labeled P. aeruginosa without the plasmid shows only green fluorescence (top row). In contrast, the GFP-labeled P. aeruginosa transformed by pCN61_AmpRtdT shows green fluorescence (constituitively) and the red fluorescence induced by the cell-wall-acting antibiotic (bottom row). The top row confirms the absence of red backgroud fluorescence from P. aeruginosa. The bottom row demonstrates the functionality of our reporter plasmid. A 10 μM scale bar is given in panel two of the top row.

    Journal: ACS chemical biology

    Article Title: Fluorescence Assessment of the AmpR-Signaling Network of Pseudomonas aeruginosa to Exposure to β ‑Lactam Antibiotics

    doi: 10.1021/acschembio.9b00875

    Figure Lengend Snippet: Schematic depiction of reporter plasmid and fluorescence gene induction by the β-lactam antibiotic. (A) The plasmid map for pCN61_AmpRtdT is shown to the left. Ampicillin (AP, Tem-1 β-lactamase) or streptomycin (STR) is used for selection. The ampRC operon (top right) and the reporter operon where tdTomato is positioned downstream of the ampC promoter (denoted PampC) are depicted. (B) We constructed GFP-labeled P. aeruginosa and transformed this bacterium with pCN61_AmpRtdT. The bacteria were inoculated on an agar plate in proximity to the β-lactam antibiotic CAZ (spotted at the red dot). The antibiotic diffuses outward from the spot. The interface (white arrow) of CAZ and the GFP-labeled P. aeruginosa without the plasmid shows only green fluorescence (top row). In contrast, the GFP-labeled P. aeruginosa transformed by pCN61_AmpRtdT shows green fluorescence (constituitively) and the red fluorescence induced by the cell-wall-acting antibiotic (bottom row). The top row confirms the absence of red backgroud fluorescence from P. aeruginosa. The bottom row demonstrates the functionality of our reporter plasmid. A 10 μM scale bar is given in panel two of the top row.

    Article Snippet: The fluorescent-reporter systems were excised from pUC57 with restriction enzymes Bam HI and Pst I and ligated into an E. coli - P. aeruginosa shuttle vector, pCN61 (ATCC).

    Techniques: Plasmid Preparation, Fluorescence, Selection, Construct, Labeling, Transformation Assay

    Correlation of the β-lactamase and fluorescence assays. (A) Nitrocefin hydrolysis by AmpC β-lactamase shifts λmax from 390 nm (yellow) to 486 nm (red). (B) Nitrocefin assay performed for P. aeruginosa wild-type and dacB::Tn is plotted as the slope of the absorbance at 486 nm over time for two concentrations of FOX (1/8 MIC and 1/4 MIC) and no antibiotic. (C) Fluorescent response expressed as relative fluorescence (A.U.) of P. aeruginosa wild-type and dacB::Tn under the same experimental conditions as used for the nitrocefin assay. The error bars represent the standard deviation of three biological replicates.

    Journal: ACS chemical biology

    Article Title: Fluorescence Assessment of the AmpR-Signaling Network of Pseudomonas aeruginosa to Exposure to β ‑Lactam Antibiotics

    doi: 10.1021/acschembio.9b00875

    Figure Lengend Snippet: Correlation of the β-lactamase and fluorescence assays. (A) Nitrocefin hydrolysis by AmpC β-lactamase shifts λmax from 390 nm (yellow) to 486 nm (red). (B) Nitrocefin assay performed for P. aeruginosa wild-type and dacB::Tn is plotted as the slope of the absorbance at 486 nm over time for two concentrations of FOX (1/8 MIC and 1/4 MIC) and no antibiotic. (C) Fluorescent response expressed as relative fluorescence (A.U.) of P. aeruginosa wild-type and dacB::Tn under the same experimental conditions as used for the nitrocefin assay. The error bars represent the standard deviation of three biological replicates.

    Article Snippet: The fluorescent-reporter systems were excised from pUC57 with restriction enzymes Bam HI and Pst I and ligated into an E. coli - P. aeruginosa shuttle vector, pCN61 (ATCC).

    Techniques: Fluorescence, Standard Deviation

    GFP-labeled P. aeruginosa harboring pCN61_AmpRtdT was imaged on a swarm plate in proximity of a second bacterium, either (A) P. mesacidophila, (B) B. licheniformis, (C) E. coli, or (D) M. xanthus. Each panel depicts the bacteria after 15 h of growth. P. aeruginosa is to the left of each plate, while the second bacterium is to the right (see plate panels). The white arrow (top plate) points to a representative site where the two bacteria encounter one another. The second column from left shows bright-field images of comingled bacteria, where the strains meet, as uniform continuous lawns. The GFP panel shows the green fluorescence displayed by P. aeruginosa in this lawn. The black voids are the locations of the second bacterium in this same lawn. The RFP panel shows the red-fluorescent signal from P. aeruginosa that results from contact with an antibiotic-producer strain. Red fluorescence is seen for P. mesacidophila and B. lichemiformis as antibiotic producers. No red fluorescence is seen for E. coli and M. xanthus (not antibiotic producers). The far-right column merges the green and red fluorescent images. A 10-μm black scale bar is given in the bright-field image of panel A.

    Journal: ACS chemical biology

    Article Title: Fluorescence Assessment of the AmpR-Signaling Network of Pseudomonas aeruginosa to Exposure to β ‑Lactam Antibiotics

    doi: 10.1021/acschembio.9b00875

    Figure Lengend Snippet: GFP-labeled P. aeruginosa harboring pCN61_AmpRtdT was imaged on a swarm plate in proximity of a second bacterium, either (A) P. mesacidophila, (B) B. licheniformis, (C) E. coli, or (D) M. xanthus. Each panel depicts the bacteria after 15 h of growth. P. aeruginosa is to the left of each plate, while the second bacterium is to the right (see plate panels). The white arrow (top plate) points to a representative site where the two bacteria encounter one another. The second column from left shows bright-field images of comingled bacteria, where the strains meet, as uniform continuous lawns. The GFP panel shows the green fluorescence displayed by P. aeruginosa in this lawn. The black voids are the locations of the second bacterium in this same lawn. The RFP panel shows the red-fluorescent signal from P. aeruginosa that results from contact with an antibiotic-producer strain. Red fluorescence is seen for P. mesacidophila and B. lichemiformis as antibiotic producers. No red fluorescence is seen for E. coli and M. xanthus (not antibiotic producers). The far-right column merges the green and red fluorescent images. A 10-μm black scale bar is given in the bright-field image of panel A.

    Article Snippet: The fluorescent-reporter systems were excised from pUC57 with restriction enzymes Bam HI and Pst I and ligated into an E. coli - P. aeruginosa shuttle vector, pCN61 (ATCC).

    Techniques: Labeling, Fluorescence